'The Official BBC Children In Need Medley', which features over 100 animated characters, was released in aid of charity.
Elsewhere in the chart, Alicia Keys reached number eight with 'Don't Mean Anything', while Timbaland and Nelly Furtado got to number nine with 'Morning After Dark'.
After her performance on last week's X Factor, Rihanna moved four places up the chart to number two with 'Russian Roulette'.
Lady GaGa's 'Bad Romance' climbed two places to number three, but Leona Lewis slipped out of the top ten to number 15 with 'Happy'.
The top ten singles in full (click for our reviews):
1. (1) Peter Kay's Animated All Star Band: 'The Official BBC Children In Need Medley'
2. (6) Rihanna: 'Russian Roulette'
3. (5) Lady GaGa: 'Bad Romance'
4. (4) Black Eyed Peas: 'Meet Me Halfway'
5. (2) 'X Factor' Finalists 2009: 'You Are Not Alone'
6. (3) Jason Derulo: 'Whatcha Say'
7. (7) Ke$ha: 'Tik Tok'
8. (-) Alicia Keys: 'Doesn't Mean Anything'
9. (-) Timbaland ft. Nelly Furtado, SoShy: 'Morning After Dark'
10. (8) Cheryl Cole: 'Fight For This Love'
Source: Official Charts Company
